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Molecular cloning of GA-suppressed G2 pea genes by cDNA RDA

Y Zhu1, Y Zhang, H Li

  • 1State Key Laboratory of Protein Engineering and Plant Genetic Engineering, Beijing, China.

Science in China. Series C, Life Sciences
|August 1, 1997
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...