Related Experiment Video
Updated: Jul 2, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time
E Biagi1, B Vitali, C Pugliese
1Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Abstract:
The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time.
Related Concept Videos
Microbiota of the Urogenital Tract
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Trichomoniasis
Human Virome

