Related Experiment Video
Updated: Jul 2, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Published on: November 1, 2016
Structural feature of sorghum chloroplastpsbA gene and regulation effects of its 5'-noncoding region
1Institute of Developmental Biology, Chinese Academy of Sciences, 100080, Beijing, China.
Abstract:
Comparative analysis reveals remarkahle homology between the sequences of bothpsbA gene nucleotides and the inferred amino acids of sorghum, a C(4) plant, and those of rice, a C(3) plant. The 5'-noncoding region of sorghumpsbA gene contains the conservative promoter elements, "-35" element and "-10" element, like the prokaryote and the promoter element, TATA box, like the eukaryote. As compared with that of the rice, an extra sequence of 7 bp is found in the leader sequence of the mRNA in the former. Using anin vitro system, it has been demonstrated that protein factor exists in sorghum chloroplast protein extract which specifically hinds to the 5'-noncoding region ofpsbA gene. Measurement of the expression of luciferase shows a 2-5 time greater reaction of the expression plasmids pALqs which contain leader region of sorghumpsbA gene than that of the expression plasmids pALqr which contain leader region of ricepsbA gene inE. coli.
Related Concept Videos
Gene Regulation During Sporulation
Prokaryotic Gene Structure and Organization
Cis-regulatory Sequences
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Regulation of Nuclear Protein Sorting
Regulation of Expression at Multiple Steps

