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Updated: Jul 2, 2026
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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
Published on: December 6, 2013
Cell surface biomarkers of embryonic stem cells
Kohji Nagano1, Yoko Yoshida, Toshiaki Isobe
1Department of Pharmaceutical Technology, Research Division, Chugai Pharmaceutical Co., Ltd., Kanagawa, Japan.
Proteomics
|September 4, 2008
Summary
Embryonic stem cells (ESCs) are diverse and require multiple molecular biomarkers for accurate classification and isolation. Understanding these biomarkers is key to unlocking their potential in regenerative medicine and drug discovery.
Area of Science:
- Stem cell biology
- Biomarker discovery
- Regenerative medicine
Background:
- Embryonic stem cells (ESCs) possess pluripotency for regenerative medicine and drug discovery.
- Current methods classify ESCs using molecular biomarkers, but ESCs exhibit heterogeneity.
- Identifying diverse subpopulations and understanding pluripotency regulation are crucial.
Purpose of the Study:
- To review studies on embryonic stem cell (ESC) biomarker discovery.
- To highlight the importance of biomarkers for classifying ESC subpopulations and monitoring differentiation.
- To explore the role of biomarkers in understanding pluripotency and differentiation mechanisms.
Main Methods:
- Genome-wide expression profiling of ESC mRNAs and proteins.
- Analysis of the ESC cell surface subproteome.
- Review of existing literature on ESC biomarker discovery.
Main Results:
- ESCs are heterogeneous, with subpopulations characterized by distinct molecular biomarkers.
- ESCs express a wide array of biomarkers, including cell surface antigens and signaling molecules.
- Key molecules ensuring stemness have been identified.
Conclusions:
- Biomarker discovery is essential for characterizing ESC heterogeneity and regulating pluripotency.
- Future quantitative proteomics will offer deeper insights into ESC proteome dynamics during differentiation.
- Advanced proteomic approaches will refine our understanding of nascent and amplified ESCs.
Related Concept Videos
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...