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Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell types have...
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
Membrane Proteins01:30

Membrane Proteins

Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
Membrane Proteins01:30

Membrane Proteins

Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...

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Updated: Jul 2, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Membrane proteins and membrane proteomics.

Sandra Tan1, Hwee Tong Tan, Maxey C M Chung

  • 1Faculty of Science, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.

Proteomics
|September 4, 2008
PubMed
Summary
This summary is machine-generated.

Biological membranes compartmentalize cells and organelles. Membrane proteomics, crucial for understanding cell functions and disease, is advancing with new technologies to overcome traditional study challenges.

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Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
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Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

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Last Updated: Jul 2, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Published on: April 20, 2015

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
08:24

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Published on: August 18, 2017

Area of Science:

  • Cell Biology
  • Biochemistry
  • Proteomics

Background:

  • Biological membranes are crucial for cellular structure and function, separating cellular compartments and the cell from its environment.
  • Membrane proteins perform vital roles in cellular processes, disease pathogenesis, and as drug targets.
  • Despite comprising 30% of predicted proteins, membrane proteomics remains understudied due to technical difficulties.

Purpose of the Study:

  • To highlight the importance of studying membrane proteins.
  • To discuss the challenges in membrane proteomics.
  • To emphasize the potential of technological advancements in overcoming these challenges.

Main Methods:

  • Review of existing literature on membrane biology and proteomics.
  • Discussion of traditional challenges in membrane protein analysis (solubilization, separation, identification).
  • Exploration of emerging technologies impacting membrane proteomics.

Main Results:

  • Membrane proteins are integral to diverse cellular functions and disease mechanisms.
  • Significant technical hurdles have historically limited membrane proteomics research.
  • Recent technological progress shows promise for advancing the field.

Conclusions:

  • Further research into membrane proteins is essential due to their critical roles.
  • Advancements in technology are expected to facilitate more comprehensive membrane proteomics studies.
  • Improved understanding of membrane proteins will impact cell biology, disease research, and drug discovery.