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A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
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Published on: March 20, 2019

Wired-enzyme core-shell Au nanoparticle biosensor.

P Scodeller1, V Flexer, R Szamocki

  • 1INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina.

Journal of the American Chemical Society
|September 4, 2008
PubMed
Summary
This summary is machine-generated.

We developed a novel glucose-responsive nanoparticle system. This biosensor uses self-assembled enzymes and osmium wires on gold nanoparticles for sensitive glucose detection.

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Area of Science:

  • Nanotechnology
  • Biosensors
  • Electrochemistry

Background:

  • Core-shell nanoparticles offer unique platforms for integrating multiple functionalities.
  • Osmium complexes are valuable for electrochemical and photonic biosensing applications.
  • Glucose oxidase is a key enzyme for glucose detection, but requires efficient signal transduction.

Purpose of the Study:

  • To create a fully integrated core-shell nanoparticle system for glucose detection.
  • To utilize self-assembled glucose oxidase and osmium molecular wires for enhanced biosensing.
  • To demonstrate both amperometric and photonic detection of glucose using the developed nanoparticle system.

Main Methods:

  • Fabrication of core-shell gold nanoparticles functionalized with glucose oxidase and osmium molecular wires.
  • Characterization using spectroscopy, quartz crystal microbalance, and electrochemical techniques.
  • Amperometric and Surface-Enhanced Raman Spectroscopy (SERS)-like detection of glucose.

Main Results:

  • Successful self-assembly of a catalytically active multilayer shell on core-shell nanoparticles.
  • Demonstrated reagentless amperometric detection of glucose in the millimolar range.
  • Achieved contactless photonic biosensing via osmium resonant Raman signal, enhanced by SERS.

Conclusions:

  • The core-shell nanoparticle system is a viable platform for glucose-responsive chemistry.
  • The integration of enzymes and osmium wires on nanoparticles advances biosensor design.
  • This approach extends glucose detection from planar surfaces to nanoparticle systems, offering enhanced signal amplification.