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Hofmeister effect and the phase diagram of lysozyme
S Lettieri1, Xiaofei Li, J D Gunton
1Department of Physics, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Abstract:
The phase diagrams for lysozyme are calculated for two different precipitant salts, NaCl and NaSCN, using a potential of mean force that takes into account contributions from ion-dispersion forces [M. Boström, J. Phys. Chem. B 110, 24757 (2006)]. Our results are consistent with a recent perturbation theory calculation (referenced above) in that the phase diagram for lysozyme with NaCl is quite different than for lysozyme with NaSCN for the same molar concentration (0.2M) . However, in contrast to the perturbation theory calculation, we find that the lysozyme phase diagram with NaCl has a metastable fluid-fluid coexistence curve and that the metastability gap in the case of NaSCN is much larger than predicted by perturbation theory.
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