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Updated: Jul 2, 2026

Preparation of Complaint Matrices for Quantifying Cellular Contraction
Published on: December 14, 2010
Insensitivity to salt of assembly of a rigid biopolymer aggrecan
Ferenc Horkay1, Peter J Basser, Anne-Marie Hecht
1Section on Tissue Biophysics and Biomimetics, Laboratory of Integrative and Medical Biophysics, NICHD, National Institutes of Health, 13 South Drive, Bethesda, Maryland 20892, USA.
Abstract:
Many polyelectrolytes, ranging from sulfonated polystyrene to DNA, exhibit a strong sensitivity of their phase behavior to salt concentration, especially to higher valence salts, which often lead to phase separation. We show that the stiff polyelectrolyte aggrecan exhibits a qualitatively different behavior. Specifically, the scattering properties of aggrecan solutions are exceptionally insensitive to the addition of calcium salt, conferring on aggrecan the role of an ion reservoir mediating calcium metabolism in cartilage and bone, and also providing osmotic resilience to compressive load.
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