Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic Insights Into the Oxidative Reactivity of 2-Selenouridine in tRNA.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Exacerbation of hemorrhagic stroke and impaired hemostasis in mice deficient of the integrated stress response kinase heme-regulated inhibitor.

Brain circulation·2026
Same author

Chalcogen-modified Nucleic Acid Analogues.

Current medicinal chemistry·2026
Same author

From U to mnm⁵Se²U: tuning base pairing preferences through 2-chalcogen and 5-methylaminomethyl modifications.

Scientific reports·2025
Same author

Limited effects of the REV-ERB agonist SR9009 after mouse spinal cord contusion: Reduced acute pathology with unaffected functional recovery and chronic white matter loss.

Neuroscience letters·2025
Same author

Biochemical and biophysical characterization, and 3D structure modeling of human HINT3, a hydrolase of the HIT superfamily.

Bioorganic chemistry·2025

Related Experiment Video

Updated: Jul 2, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

S100A6 binds p53 and affects its activity.

Łukasz P Słomnicki1, Barbara Nawrot, Wiesława Leśniak

  • 1Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093 Warsaw, Poland.

The International Journal of Biochemistry & Cell Biology
|September 4, 2008
PubMed
Summary
This summary is machine-generated.

S100A6 (calcyclin) protein binds to p53, enhancing its transcriptional activity and promoting apoptosis. This interaction influences p53

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Related Experiment Videos

Last Updated: Jul 2, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • S100A6 (calcyclin) is a calcium-binding protein involved in numerous cellular functions.
  • S100A6 is frequently overexpressed in various cancers.
  • Identifying S100A6 targets is crucial for understanding its biological roles.

Purpose of the Study:

  • To identify protein targets of S100A6.
  • To investigate the functional consequences of S100A6 interaction with p53.

Main Methods:

  • Affinity chromatography and co-immunoprecipitation were used to detect S100A6-p53 interaction.
  • Small interfering RNA (siRNA) was employed to reduce S100A6 levels in HEp-2 cells.
  • Electrophoretic mobility shift assay (EMSA) assessed p53 DNA binding.
  • p53 transcriptional activity and nuclear accumulation were evaluated.

Main Results:

  • S100A6 was found to interact with p53 in a calcium-dependent manner.
  • Reduced S100A6 levels led to decreased p53 transcriptional activity and apoptosis.
  • S100A6 enhances p53 nuclear accumulation under stress but does not affect DNA binding.
  • S100A6 presence correlates with increased susceptibility to hydrogen peroxide-induced apoptosis.

Conclusions:

  • S100A6 directly interacts with p53.
  • S100A6 modulates p53 activity, enhancing its transcriptional function and nuclear localization.
  • The S100A6-p53 interaction plays a role in cellular responses to stress and apoptosis.