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Updated: Jul 2, 2026
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Published on: January 6, 2017
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein
Shu Wang1, Yong Xing He, Rui Bao
1School of Life Sciences and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, People's Republic of China.
Abstract:
A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 A resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 29.67, b = 67.00, c = 34.43 A, alpha = gamma = 90.00, beta = 94.26 degrees .
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