Related Experiment Video
Updated: Jul 2, 2026
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Serial propagation and purification of chicken anaemia agent in MDCC-MSB1 cell line
1Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Tottori-shi, Tottori, Japan.
Abstract:
The TK-5803 strain of an agent which induces chicken anaemia was propagated and titrated in cultures of the MDCC-MSB1 cell line. When the isolates (tentatively designated MSBI-TK-5803 strain) from the original material were inoculated into 1-day-old susceptible chicks, they showed a severe pathogenicity, inducing anaemia with aplasia of the bone marrow and atrophy of the lymphoid organs. On density gradient separation, the peak of their infectivity titre appeared at a density of 1.35 to 1.36 g/cm(3) and numerous virus particles were demonstrated in the same fraction. The agent was resistant to heating for 30 min at 60 degrees C and 50% chloroform for 15 min at 4 degrees C, and passed through a 25 nm membrane filter, but it was completely inactivated after heating for 30 min at 70 degrees C or more. The purified virus particles were spherical or hexagonal in shape and 19.1 +/- 0.2 nm in diameter.
Insights
A novel strain of chicken anemia virus (CAV), MSBI-TK-5803, was isolated and characterized. This virulent strain causes severe anemia and lymphoid organ atrophy in young chicks, highlighting its pathogenic potential.
Area of Science:
- Virology
- Avian Pathology
Background:
- Chicken anemia virus (CAV) is an economically significant pathogen in poultry.
- Understanding CAV strains is crucial for developing effective control strategies.
Purpose of the Study:
- To propagate and characterize a newly isolated CAV strain (TK-5803).
- To assess the pathogenicity and physical properties of the MSBI-TK-5803 strain.
Main Methods:
- Propagation and titration in MDCC-MSB1 cell line.
- Inoculation of 1-day-old susceptible chicks to evaluate pathogenicity.
- Density gradient separation to determine infectivity peak.
- Physicochemical stability tests (heat, chloroform).
- Electron microscopy for particle morphology and size determination.
Main Results:
- The MSBI-TK-5803 strain induced severe anemia, bone marrow aplasia, and lymphoid organ atrophy in chicks.
- Peak infectivity was observed at a density of 1.35-1.36 g/cm(3).
- The virus demonstrated resistance to 60°C for 30 min and chloroform, but was inactivated at 70°C.
- Purified virus particles were spherical/hexagonal, measuring 19.1 ± 0.2 nm in diameter.
Conclusions:
- The MSBI-TK-5803 strain is a highly pathogenic variant of chicken anemia virus.
- Its physical and chemical properties provide insights for virus inactivation and detection methods.
- This characterization aids in understanding CAV pathogenesis and disease management in poultry.
