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Related Experiment Video

Updated: Jul 2, 2026

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Published on: August 25, 2018

Intraocular ossification in the rd chicken.

K C Kelley¹, R J Ulshafer, E A Ellis

¹Department of Ophthalmology, J. Hillis Miller Health Center, University of Florida, Gainesville, Florida 32610, USA.

Avian Pathology : Journal of the W.V.P.A

|January 1, 1987

Summary

Intraocular ossification, a bone-like condition, was observed in rd/rd chickens by 18 months. By 36 months, affected eyes showed complete ossification, unlike heterozygous chickens.

Area of Science:

Ophthalmology
Histology
Biochemistry

Background:

Intraocular ossification is a rare condition affecting the eye.
The rd chicken model provides a unique opportunity to study this phenomenon.

Purpose of the Study:

To investigate the progression and characteristics of intraocular ossification in rd/rd chickens.
To compare ossification in homozygous (rd/rd) and heterozygous (+/rd) chickens.

Main Methods:

Light, transmission electron, and scanning electron microscopy were used to examine ocular tissues.
Histological staining identified collagen and calcium.
Flame atomic absorption spectrophotometry analyzed the elemental composition of ossified vitreous.

Main Results:

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Intraocular ossification was evident in rd/rd chickens from 18 months of age.
Complete ossification of one eye and partial ossification of the other occurred in 24-36 month old rd/rd chickens.
No ossification was observed in heterozygous (+/rd) chickens at any age.
Ossified vitreous contained collagen and calcium, with elemental composition (Ca, P, Mg) similar to bone.

Conclusions:

The rd chicken model exhibits progressive intraocular ossification.
The condition affects homozygous rd/rd chickens, sparing heterozygotes.
The ossified vitreous shares biochemical similarities with bone tissue.