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Updated: Jul 2, 2026
Influenza A Virus Studies in a Mouse Model of Infection
Published on: September 7, 2017
Antigenic variation in avian paramyxovirus type 3 isolates detected by mouse monoclonal antibodies
C Anderson1, R Kearsley, D J Alexander
1Department of Microbiology and Parasitology, Royal Veterinary College, Royal College St, London, England.
Abstract:
Six mouse monoclonal antibodies raised against PMV-3/turkey/England/ MPH/81 were used to assess relationships between PMV-3 viruses isolated from exotic birds and from domestic turkeys. Twelve PMV-3 isolates from birds held in quarantine in Great Britain and nine isolates from exotic birds in Netherlands, USA, Belgium, France and F.R. Germany were inhibited by only one monoclonal antibody, 50/1412, in haemagglutination inhibition tests. In contrast PMV-3 isolates from turkeys in England and France were inhibited to high titres by all six monoclonal antibodies. Two isolates from North American turkeys and one from a turkey poult in the Federal Republic of Germany were inhibited only by monoclonal antibody 50/1412 and, to low titres, by one other monoclonal antibody. Monoclonal antibody, 50/1412, showed no inhibition of 12 varied PMV-1 strains or representatives of other avian paramyxovirus sero-types and may be useful in the differential diagnosis of PMV-3 viruses.
Insights
Monoclonal antibodies revealed distinct groups of avian Paramyxovirus type 3 (PMV-3). One antibody, 50/1412, specifically identified PMV-3 strains from exotic birds and certain turkey isolates, aiding in differential diagnosis.
Area of Science:
- Virology
- Immunology
- Avian Health
Background:
- Avian Paramyxovirus type 3 (PMV-3) circulates in various bird populations, including domestic turkeys and exotic species.
- Understanding the antigenic relationships between different PMV-3 isolates is crucial for disease surveillance and control.
- Monoclonal antibodies offer high specificity for differentiating viral subtypes.
Purpose of the Study:
- To investigate the antigenic diversity of PMV-3 isolates from domestic turkeys and exotic birds.
- To evaluate the utility of specific monoclonal antibodies in distinguishing PMV-3 strains.
- To assess the potential of monoclonal antibody 50/1412 in the differential diagnosis of PMV-3.
Main Methods:
- Six mouse monoclonal antibodies were generated against a reference PMV-3 strain (PMV-3/turkey/England/MPH/81).
- Haemagglutination inhibition (HI) tests were performed using these monoclonal antibodies against 21 PMV-3 isolates from exotic birds and 12 isolates from domestic turkeys.
- Reactions were also tested against PMV-1 strains and other avian paramyxovirus serotypes.
Main Results:
- A single monoclonal antibody, 50/1412, specifically inhibited PMV-3 isolates from exotic birds (Netherlands, USA, Belgium, France, F.R. Germany) and some turkey isolates.
- PMV-3 isolates from English and French turkeys reacted strongly with all six monoclonal antibodies.
- North American turkey isolates and one German turkey poult isolate showed limited reactivity with 50/1412 and one other antibody.
- Monoclonal antibody 50/1412 did not inhibit PMV-1 strains or other avian paramyxoviruses.
Conclusions:
- Monoclonal antibody 50/1412 demonstrates high specificity for a distinct group of PMV-3 viruses, differentiating them from strains found in domestic turkeys.
- The findings suggest antigenic variation within PMV-3, with potential implications for epidemiological studies.
- Monoclonal antibody 50/1412 shows promise as a diagnostic tool for the specific identification of certain PMV-3 strains.
