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Indirect immunofluorescence as a diagnostic tool for parvovirus infection of broiler chickens

J Kisary1

  • 1Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.

Avian Pathology : Journal of the W.V.P.A
|April 1, 1985
PubMed

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Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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