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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Strategies for profiling microRNA expression.

William Kong1, Jian-Jun Zhao, Lili He

  • 1Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, USA.

Journal of Cellular Physiology
|September 4, 2008
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) regulate cell processes and gene expression. miRNA profiling identifies expression changes in diseases, aiding in understanding normal and abnormal cellular functions.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
  • Aberrant miRNA expression is implicated in various diseases, including cancer and heart disease.
  • Thousands of human miRNAs are predicted, with over 540 currently validated.

Purpose of the Study:

  • To review current strategies for miRNA expression profiling.
  • To discuss the sensitivity, specificity, advantages, and disadvantages of these methods.

Main Methods:

  • Literature review of miRNA profiling techniques.
  • Analysis of existing data on miRNA expression studies.
  • Comparative assessment of different profiling platforms.

Main Results:

  • Various miRNA profiling strategies exist, each with unique strengths and weaknesses.
  • Profiling is crucial for identifying differential miRNA expression in health and disease.
  • Continued research is needed to validate predicted miRNAs.

Conclusions:

  • miRNA profiling is an essential tool for biomedical research.
  • Understanding miRNA expression is key to diagnosing and treating complex diseases.
  • The field requires standardized and sensitive profiling methods.