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Related Concept Videos

Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Eukaryotic ribosomes host PKC activity.

Stefano Grosso1, Viviana Volta, Marina Vietri

  • 1Laboratory of Molecular Histology and Cell Growth, DIBIT, Via Olgettina 58, HSR, 20132 Milano, Italy.

Biochemical and Biophysical Research Communications
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

Active protein kinase C (PKC) is present on ribosomes, enabling the phosphorylation of ribosome-associated proteins. This finding demonstrates a novel regulatory mechanism for translation.

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Published on: January 7, 2013

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Protein kinase C (PKC) isoform betaII interacts with ribosomes via RACK1.
  • The presence and activity of PKC on ribosomes remain unconfirmed.

Purpose of the Study:

  • To investigate whether active PKC is associated with native ribosomes.
  • To determine if ribosomes can phosphorylate substrates in a PKC-dependent manner.

Main Methods:

  • Purification of native ribosomes using two distinct techniques.
  • Assay of ribosome-associated kinase activity using MARCKS as a substrate.
  • Treatment with PKC agonist PMA and inhibitor BMI to confirm PKC involvement.

Main Results:

  • Purified native ribosomes exhibited MARCKS phosphorylation activity.
  • PMA treatment enhanced MARCKS phosphorylation, while BMI treatment abrogated it.
  • These results confirm the presence of active PKC on ribosomes.

Conclusions:

  • Native ribosomes host active protein kinase C (PKC).
  • Ribosome-associated PKC can phosphorylate substrates like initiation factors and mRNA-binding proteins.
  • This suggests a novel mechanism for regulating translation.