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Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
Published on: November 17, 2020
Sand flies, Leishmania, and transcriptome-borne solutions
Fabiano Oliveira1, Ryan C Jochim, Jesus G Valenzuela
1National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA.
Abstract:
Sand fly-parasite and sand fly-host interactions play an important role in the transmission of leishmaniasis. Vector molecules relevant for such interactions include midgut and salivary proteins. These potential targets for interruption of propagation of Leishmania parasites have been poorly characterized. Transcriptomic analysis has proven to be an effective tool for identification of new sand fly molecules, providing exciting new insights into vector-based control strategies against leishmaniasis.
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