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Updated: Jul 2, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Partitioned dispersive liquid-liquid microextraction: an approach for polar organic compounds extraction from aqueous
Mahaveer B Melwanki1, Ming-Ren Fuh
1Department of Chemistry, Soochow University, Taipei, Taiwan.
Abstract:
Partitioned dispersive liquid-liquid microextraction (PDLLME) efficiency was demonstrated for the extraction of polar organic compounds (chlorophenoxyacetic acids) prior to high performance liquid chromatography (HPLC). The method was based on the formation of tiny droplets of an organic extractant in an aqueous sample (river water) by injecting a mixture of a water-immiscible organic solvent [tetrachloroethylene (TCE)] as extractant dissolved in a water-miscible organic dispersive solvent [tetrahydrofuran (THF)]. Based on their partition coefficients, polar compounds were extracted into the dispersed TCE droplets as well as into THF. Different parameters affecting the extraction efficiency were evaluated and precision, linearity, detection limit and an enrichment factor were determined.
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