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Updated: Jul 2, 2026
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Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Sphingobacterium anhuiense sp. nov., isolated from forest soil.
1Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
International Journal of Systematic and Evolutionary Microbiology
|September 5, 2008
Summary
A novel Gram-negative bacterium, Sphingobacterium anhuiense sp. nov. strain CW 186(T), was discovered in Chinese forest soil. This bacterium represents a new species within the Sphingobacterium genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Sphingobacterium comprises Gram-negative bacteria with diverse ecological roles.
- Accurate bacterial classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To characterize a novel bacterial isolate from forest soil.
- To determine the taxonomic position of the isolate within the genus Sphingobacterium.
Main Methods:
- Isolation and cultivation of bacteria from forest soil.
- Morphological, physiological, and biochemical characterization.
- Chemotaxonomic analysis (fatty acids, isoprenoid quinones).
- Phylogenetic analysis based on 16S rRNA gene sequencing.
Main Results:
- Strain CW 186(T) is a strictly aerobic, non-motile, rod-shaped, Gram-negative bacterium.
- Optimal growth at 25–30°C and pH 6.0–8.0.
- Characteristic fatty acid profile (iso-C15:0, iso-C17:0 3-OH, summed feature 3) and MK-7 quinone.
- 16S rRNA gene sequence analysis revealed <94% similarity to known Sphingobacterium species, with highest similarity (93.9%) to Sphingobacterium composti.
- Genomic G+C content was 36.3 mol%.
Conclusions:
- Strain CW 186(T) represents a distinct species within the genus Sphingobacterium.
- The novel species is named Sphingobacterium anhuiense sp. nov., with strain CW 186(T) as the type strain.
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