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Related Experiment Videos

Acrolein as a fixative for enzyme cytochemistry.

T Saito, H Keino

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |December 1, 1976
    PubMed
    Summary

    Acrolein is a viable prefixative for enzyme ultracytochemistry, preserving enzyme activity even in sensitive tissues. Its rapid penetration allows for larger tissue blocks, benefiting pathological specimen selection.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Histology

    Background:

    • Aldehyde fixation is crucial for preserving cellular structures and enzyme activity in cytochemistry.
    • Glutaraldehyde is a common fixative but has limitations in penetration depth.
    • Acrolein's rapid penetration offers potential advantages for tissue fixation.

    Purpose of the Study:

    • To reinvestigate acrolein as a prefixative in enzyme cytochemistry.
    • To evaluate acrolein's efficacy in preserving enzyme activities, particularly vulnerable ones.
    • To assess the combined use of acrolein and glutaraldehyde.

    Main Methods:

    • Enzyme cytochemistry techniques were employed.
    • Tissues were fixed using acrolein at low concentrations.
    • Enzyme activities, including glucose-6-phosphatase, were measured.
    • Comparative fixation with glutaraldehyde and a combination of both aldehydes was performed.

    Main Results:

    • Acrolein at low concentrations effectively preserves enzyme activities, including glucose-6-phosphatase.
    • Enzyme activities are maintained in tissues fixed with both acrolein and glutaraldehyde.
    • Acrolein's rapid penetration allows fixation of larger tissue blocks.

    Conclusions:

    • Acrolein is suitable for enzyme ultracytochemistry due to its ability to preserve enzyme activity.
    • The combination of acrolein and glutaraldehyde also yields preserved enzyme activities.
    • Acrolein's properties enhance specimen selection, especially in pathology.

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