A plaque assay for duck hepatitis virus.
P R Woolcock1, W S Chalmers, D Davis
1Duck Virus Unit, Animal Health Trust, Small Animals Centre, Kennett, Newmarket, Suffolk, England.
Avian Pathology : Journal of the W.V.P.A
|January 1, 1982
Summary
A new plaque assay for duck hepatitis virus (DHV) was developed using primary duck embryo kidney cells. The assay
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Duck hepatitis virus (DHV) is a significant pathogen affecting young ducks.
- Accurate methods for quantifying DHV are crucial for research and diagnostics.
Purpose of the Study:
- To describe and validate a plaque assay for DHV.
- To assess key performance characteristics of the assay.
Main Methods:
- Primary duck embryo kidney cell monolayers were used.
- A plaque assay was developed and optimized.
- Parameters including repeatability, sensitivity, and specificity were evaluated.
Main Results:
- The plaque assay provides a reproducible method for DHV quantification.
- The assay demonstrated acceptable sensitivity and specificity.
Conclusions:
- The described plaque assay is a reliable tool for studying DHV.
- This method can aid in the diagnosis and control of duck hepatitis.
