Survival of Mycoplasma gallisepticum in mains water

The survival of two strains of Mycoplasma gallispeticum in mains water at room temperature depended on the initial number of organisms and the incorporation of mycoplasma broth in the suspending medium. Seven to 8 log(10) colony forming units (cfu) ml were needed for survival for 1 day in sterile water. In water with 1% (vol/vol) broth at least 6 log(10 )cfu/ml were needed for survival, which was prolonged to 4 days. In water with 10% broth 1 to 4 log(10) cfu/ml could survive for 9 to 10 days, which is similar to the survival time of low concentrations of mycoplasma in pure broth.