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Updated: Jul 2, 2026

Methods for Detecting Cough and Airway Inflammation in Mice
Published on: August 2, 2024
[Immune phenomena in experimental syngamosis. 1. Antigenic structure of Syngamus trachea]
A F Petavy1, T Duriez-Vaucelle, A Vernes
1U 42 de l'Inserm et du Laboratoire de Parasitologie de la Faculte de Médecine de Lille, France.
The soluble somatic antigens of Syngamus trachea were studied by gel electrophoresis and by immunoelectrophoresis, including staining for enzyme activity. Rabbit antiserum prepared by repeated injections of parasite material was used to demonstrate the antigens. Serum obtained after 12-15 weekly injections produced up to 16 precipitation arcs with the S. trachea preparation. Two of the arcs represented reactions against contaminating host material, and enzyme activity was detected in seven arcs. Cross reactions occurred with extracts of Trichinella spiralis (two arcs) and Ascaris lumbricoides (one arc).
The soluble somatic antigens of Syngamus trachea were studied by gel electrophoresis and by immunoelectrophoresis, including staining for enzyme activity. Rabbit antiserum prepared by repeated injections of parasite material was used to demonstrate the antigens. Serum obtained after 12-15 weekly injections produced up to 16 precipitation arcs with the S. trachea preparation. Two of the arcs represented reactions against contaminating host material, and enzyme activity was detected in seven arcs. Cross reactions occurred with extracts of Trichinella spiralis (two arcs) and Ascaris lumbricoides (one arc).
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