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Updated: Jul 2, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS)
Christophe Lacomme1, Sean Chapman
1Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom.
Abstract:
Potato virus X-based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down-regulate genes through virus-induced gene silencing. The development of binary-based vectors for Agrobacterium delivery makes this system well suited to high-throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro-grown plant material.

