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Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
Published on: January 24, 2025
Laboratory maintenance of Treponema denticola
1University of Michigan School of Dentistry, Ann Arbor, Michigan, USA.
Current Protocols in Microbiology
|September 5, 2008
Abstract:
This unit describes the methods, media, and equipment necessary for routine laboratory culture and handling of the anaerobic oral spirochete Treponema denticola. Topics discussed include nutrient requirements, recommended media formulations, and expected growth kinetics, as well as methods and equipment necessary to maintain anaerobic conditions. An additional protocol on isolation of T. denticola from clinical samples is included.
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