Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Published on: January 15, 2011

Growing and analyzing biofilms in flow cells.

Claus Sternberg¹, Tim Tolker-Nielsen

¹The Technical University of Denmark, Lyngby, Denmark.

Current Protocols in Microbiology

|September 5, 2008

Summary

This unit details flow cell systems for studying microbial biofilms, enabling direct microscopic analysis of structural development under controlled hydrodynamic conditions. Protocols cover system setup, operation, and advanced techniques like fluorescent in situ hybridization for biofilm research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Peroxidase-mimicking chitosan/dextran-coated cobalt ferrite-graphene oxide nanozyme enhances hydrogen peroxide-mediated killing of cariogenic pathogens in dental biofilm models.

Scientific reports·2026

Same author

Combining Yeast Display and Bacterial Genomic Library for the Unbiased Isolation of Novel Polysaccharide-Binding Peptides.

International journal of molecular sciences·2026

Same author

Biofilm-associated molecular patterns: BAMPs.

Infection and immunity·2025

Same author

Small molecule antipathogenic agents against <i>Staphylococcus aureus</i> infections.

RSC medicinal chemistry·2025

Same author

Co-culture biofilm patterns among different Pseudomonas aeruginosa clones from cystic fibrosis patients.

Biofilm·2025

Same author

Enhanced penetration and biofilm eradication by sophorolipid micelles encapsulating Honokiol: a comprehensive solution for biofilm-associated lung infections.

Journal of nanobiotechnology·2025

Area of Science:

Microbiology
Biotechnology
Microscopy

Background:

Microbial biofilms are complex structures crucial in various environments.
Studying biofilm development requires advanced imaging and controlled conditions.
Traditional methods may not fully capture dynamic biofilm formation.

Purpose of the Study:

To describe the setup and use of flow cell systems for microbial biofilm research.
To provide detailed protocols for analyzing structural biofilm development.
To enable direct microscopic investigation of biofilms under controlled hydrodynamic conditions.

Main Methods:

Construction and assembly of flow cell systems, including bubble traps.
Sterilization, inoculation, and operation of the flow cell system.

More Related Videos

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Published on: March 11, 2015

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Published on: October 18, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Published on: January 15, 2011

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Published on: March 11, 2015

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Published on: October 18, 2018

Image capture, analysis, embedding, and fluorescent in situ hybridization of biofilms.

Main Results:

Flow cell systems allow for direct, real-time microscopic observation of biofilm formation.
Environmental conditions within the flow cell can be precisely controlled and modified.
Detailed protocols facilitate reproducible biofilm studies.

Conclusions:

Flow cell technology is a powerful tool for investigating microbial biofilm structure and development.
The described methods enable comprehensive analysis of biofilms under dynamic conditions.
This unit provides a valuable resource for researchers studying biofilm dynamics.