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Related Experiment Video

Updated: Jul 2, 2026

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
07:24

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

Published on: May 25, 2022

Newcastle disease virus: propagation, quantification, and storage.

Lori W McGinnes1, Homer Pantua1, Julie Reitter1

  • 1University of Massachusetts Medical School, Worcester, Massachusetts.

Current Protocols in Microbiology
|September 5, 2008
PubMed
Summary

Newcastle disease virus (NDV) preparation methods are crucial for research and vaccines. Growing NDV in embryonated chicken eggs yields infectious virus, unlike tissue culture methods.

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Published on: October 11, 2013

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Newcastle disease virus (NDV) is a prototype paramyxovirus.
  • NDV is vital for studying viral life cycles and pathogenicity.
  • Some NDV strains are virulent poultry pathogens, while others are used as vaccines.

Purpose of the Study:

  • To describe essential methods for preparing and titrating Newcastle disease virus (NDV) stocks.
  • To compare NDV growth in embryonated chicken eggs versus tissue culture.
  • To outline standard titration assays for NDV.

Main Methods:

  • NDV stocks were grown and purified using embryonated chicken eggs.
  • NDV stocks were also prepared using tissue culture cells.
  • Virus stocks were titrated using plaque assays and hemagglutination assays.

Main Results:

  • Embryonated chicken eggs provide a superior method for growing infectious NDV stocks across all strains.
  • Avirulent NDV stocks grown in tissue culture were found to be noninfectious.
  • Plaque and hemagglutination assays are effective for routine NDV stock titration.

Conclusions:

  • Standardized methods for NDV stock preparation and titration are essential for research and vaccine development.
  • Embryonated chicken eggs are the preferred method for generating infectious NDV stocks.
  • Tissue culture methods may yield noninfectious stocks for certain NDV strains.