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Related Concept Videos

Influenza01:27

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...

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Updated: Jul 2, 2026

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
09:02

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Published on: February 20, 2021

Influenza: propagation, quantification, and storage.

Kristy J Szretter1, Amanda L Balish, Jacqueline M Katz

  • 1Influenza Branch, Centers for Disease, Control and Prevention, Atlanta, Georgia, USA.

Current Protocols in Microbiology
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

Influenza A and B viruses are major human pathogens. Madin-Darby Canine Kidney (MDCK) cells are the preferred choice for isolating these influenza viruses from clinical samples.

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Published on: March 19, 2015

Area of Science:

  • Virology
  • Microbiology

Background:

  • Influenza viruses are RNA viruses responsible for seasonal epidemics.
  • Influenza A and B are the primary types causing human illness.
  • Influenza A is further classified into subtypes based on hemagglutinin (HA) and neuraminidase (NA) proteins.

Purpose of the Study:

  • To identify the optimal cell lines for influenza virus isolation.
  • To highlight the importance of host and clinical information in virus isolation.

Main Methods:

  • Review of influenza virus classification and characteristics.
  • Discussion of cell line utility for viral isolation.

Main Results:

  • MDCK cells are identified as the gold standard for isolating human influenza A and B viruses.
  • The choice of cell line is influenced by the specimen's origin and clinical context.

Conclusions:

  • MDCK cells provide reliable isolation of human influenza viruses.
  • Accurate influenza virus identification relies on appropriate cell line selection and consideration of clinical data.