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Related Experiment Video

Updated: Jul 2, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Human immunodeficiency viruses: propagation, quantification, and storage.

Kathryn H Richards1, Paul R Clapham

  • 1University of Massachusetts, Medical School, Worcester, Massachusetts, USA.

Current Protocols in Microbiology
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

This unit details essential protocols for human immunodeficiency virus (HIV) research, covering propagation, quantification, and storage methods. It offers background, advantages, disadvantages, and troubleshooting for these frequently used techniques.

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human Immunodeficiency Virus (HIV) research is critical for understanding and combating the global epidemic.
  • Standardized and reliable laboratory protocols are essential for reproducible HIV research.
  • Existing methods require clear documentation of advantages, disadvantages, and troubleshooting for effective implementation.

Purpose of the Study:

  • To provide a comprehensive guide to fundamental laboratory protocols for HIV research.
  • To detail methods for HIV propagation and quantification.
  • To offer recommendations for the long-term storage of HIV and related materials.

Main Methods:

  • Description of established protocols for propagating HIV in cell cultures.
  • Explanation of quantitative assays for measuring HIV levels (e.g., viral load).
  • Guidelines for optimal cryopreservation and storage of HIV stocks and samples.

Main Results:

  • The unit presents detailed protocols with clear instructions.
  • Advantages and disadvantages of each method are discussed.
  • Common issues and troubleshooting tips are provided for each protocol.

Conclusions:

  • Standardized protocols enhance the reliability and reproducibility of HIV research.
  • Effective propagation, quantification, and storage are foundational for experimental success.
  • This guide serves as a valuable resource for researchers working with HIV.