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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
Published on: July 12, 2013
Genetic Manipulation of Mycobacterium tuberculosis
Michelle H Larsen1, Karolin Biermann, Steven Tandberg
1Albert Einstein College of Medicine, Bronx, New York, USA.
Current Protocols in Microbiology
|September 5, 2008
Abstract:
This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed.
