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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
Epstein-Barr Virus (EBV): infection, propagation, quantitation, and storage
Ke Lan1, Subhash C Verma, Masanao Murakami
1University of Pennsylvania Medical School, Philadelphia, Pennsylvania, USA.
Current Protocols in Microbiology
|September 5, 2008
Summary
Epstein-Barr virus (EBV) causes various cancers and diseases. This study details methods for preparing, quantifying, and storing EBV for research into its infection and oncogenesis mechanisms.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Epstein-Barr virus (EBV) is linked to Burkitt's lymphoma, nasopharyngeal carcinoma, and other malignancies.
- EBV is also associated with infectious mononucleosis and AIDS complications.
- EBV's role in T cell lymphomas, Hodgkin's disease, and transplant-related B cell lymphomas is established.
Purpose of the Study:
- To describe established procedures for EBV virion preparation.
- To outline methods for in vitro infection of cells with EBV.
- To provide protocols for EBV quantitation and storage.
Main Methods:
- Preparation of EBV virion particles.
- In vitro infection of cells using EBV.
- Quantitation and storage of EBV.
Main Results:
- Established EBV-infected cell lines can produce infectious viral progeny.
- These cell lines facilitate the study of EBV infection, transformation, and oncogenesis.
- Standardized procedures for EBV preparation and handling are provided.
Conclusions:
- The described methods enable further research into EBV-associated diseases.
- Standardized protocols are crucial for reproducible EBV research.
- Understanding EBV mechanisms can lead to new therapeutic strategies.
