Optical filter sets for multiparameter flow cytometry

One of the most important components of a fluorescence detection system, the optical filter set selects wavelengths of light for fluorophore excitation and fluorescent signal discrimination. Of the variety of optical devices available for wavelength selection, optical filters have gained wide acceptance because of their wavelength selectivity, ease of installation, and relative inexpensiveness. This unit discusses the properties, compositions, and uses of optical filters in flow cytometry. The information should allow investigators to select quality filters for modifying systems for new dyes and multicolor analyses.