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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 2, 2026

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Flow cytometry of yeasts.

¹University of Wales, Cardiff, United Kingdom.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Flow cytometry is a valuable tool for analyzing yeast cell populations. This unit details methods for assessing cell cycle, viability, and enzyme activity in yeasts, enhancing their biological research applications.

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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Area of Science:

Biotechnology
Microbiology
Cell Biology

Background:

Flow cytometry is a powerful technique for analyzing cellular characteristics.
While widely used in various biological systems, detailed protocols for yeast applications are less common.
Advancements in digital imaging exist, but flow cytometry excels at resolving population heterogeneity.

Purpose of the Study:

To promote the application of flow cytometry in new research areas, specifically for yeast.
To provide detailed protocols for key cellular measurements in yeast using flow cytometry.
To highlight the continued importance of flow cytometry for analyzing heterogeneous cell populations.

Main Methods:

Detailed protocols for flow cytometry measurements.
Assays for cell cycle analysis.
Measurements of cell viability, respiratory activity, and beta-galactosidase activity in yeasts.

Main Results:

Established detailed flow cytometry protocols for yeast.
Successfully measured cell cycle, viability, respiratory, and beta-galactosidase activity in yeasts.
Demonstrated the utility of flow cytometry for resolving heterogeneity in yeast populations.

Conclusions:

Flow cytometry is an effective and essential method for detailed analysis of yeast populations.
The provided protocols facilitate the use of flow cytometry in diverse yeast research.
Flow cytometry remains superior to digital imaging for analyzing cellular heterogeneity in yeast.