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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 2, 2026

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Antibiotic susceptibility testing by flow cytometry.

D J Mason1, F C Mortimer, V A Gant

  • 1University of Warwick, Coventry, United Kingdom.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

Flow cytometry offers rapid antibiotic susceptibility testing by using fluorescent dyes to detect changes in bacterial populations. Different probes are needed for various organisms, targeting membrane or nucleic acid alterations.

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Last Updated: Jul 2, 2026

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Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Antibiotic resistance is a growing global health concern, necessitating rapid diagnostic methods.
  • Traditional antibiotic susceptibility testing can be time-consuming, delaying appropriate treatment.
  • Flow cytometry presents a potential avenue for faster assessment of antibiotic effects.

Purpose of the Study:

  • To describe fluorescent dye-based methods for assessing antibiotic susceptibility using flow cytometry.
  • To highlight the ability of flow cytometry to differentiate heterogeneous responses within microbial populations exposed to antibiotics.
  • To discuss probes targeting membrane integrity and nucleic acid alterations for flow cytometry analysis.

Main Methods:

  • Utilizing various fluorescent dyes to probe cellular changes in microbial populations.
  • Employing flow cytometry to rapidly analyze dye fluorescence and distinguish cellular states.
  • Investigating probes that detect alterations in membrane structure and nucleic acid content.

Main Results:

  • Flow cytometry can rapidly indicate antibiotic susceptibility by detecting population heterogeneity.
  • Different fluorescent probes are required to assess antibiotic impact on diverse organisms.
  • Observed changes include alterations in membrane integrity and nucleic acid characteristics.

Conclusions:

  • Flow cytometry is a valuable tool for rapid antibiotic susceptibility determination.
  • The choice of fluorescent probes is organism- and antibiotic-dependent.
  • Monitoring membrane and nucleic acid changes provides insights into antibiotic mechanisms.