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Related Experiment Video

Updated: Jul 2, 2026

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Enumeration of absolute cell counts using immunophenotypic techniques.

F Mandy¹, B Brando

¹Health Canada, Ottawa, Canada.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Absolute cell counting using flow cytometry is crucial for monitoring HIV, assessing immunodeficiency, and checking blood products. This method precisely enumerates target cells, offering accurate clinical insights.

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Area of Science:

Clinical immunology
Hematology
Biotechnology

Background:

Accurate cell enumeration is vital for managing HIV/AIDS, immune disorders, and ensuring blood product safety.
Traditional methods face challenges in precision and efficiency for absolute cell counts.

Purpose of the Study:

To present detailed protocols for absolute cell counting using flow cytometry.
To highlight the single-platform method as the preferred approach for cell enumeration.

Main Methods:

Utilizes flow cytometry as a single platform for absolute cell enumeration.
Employs electronic gating algorithms with lineage-specific immunofluorescent markers for precise cell identification.
Describes protocols for both volumetric and flow-rate determination of residual white blood cells and leukocyte subsets.

Main Results:

The single-platform flow cytometry method enables precise and automatic exclusion of unwanted cells.
Provides validated protocols for accurate enumeration of residual white blood cells and leukocyte subsets.

Conclusions:

Single-platform flow cytometry is the method of choice for absolute cell enumeration in clinical settings.
The presented protocols facilitate reliable monitoring of disease status and immune function.