Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of circulating miRNAs as biomarkers in idiopathic pulmonary arterial hypertension: possible relevance of miR-23a.

Oxidative medicine and cellular longevity·2015

Same author

New insights into cell cycle and DNA damage response machineries through high-resolution AMICO quantitative imaging cytometry.

Cell proliferation·2013

Same author

Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2013

Same author

Cytometry of apoptosis. Historical perspective and new advances.

Experimental oncology·2012

Same author

Bosentan inhibits cigarette smoke-induced endothelin receptor expression in pulmonary arteries.

The European respiratory journal·2011

Same author

DNA strand breaks occurring during apoptosis - their early insitu detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors.

International journal of oncology·2011

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Overview of nucleic acid analysis.

Z Darzynkiewicz¹, G Juan

¹New York Medical College, Valhalla, New York, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

Nucleic acid analysis via flow cytometry is a common technique in basic and clinical research, especially for oncology. These methods offer insights into cell attributes linked to tumor malignancy and molecular biology.

Area of Science:

Molecular Biology
Cell Biology
Oncology

Background:

Flow cytometry is a widely used technique in biological research.
Nucleic acid analysis is a common application of flow cytometry.
Understanding cell attributes is crucial in oncology research.

Purpose of the Study:

To discuss the broad applicability of nucleic-acid analytical methods.
To highlight the importance of these methods in basic and clinical research.
To emphasize their role in oncology and molecular biology.

Main Methods:

Flow cytometry techniques for nucleic acid analysis.
Methodologies for assessing cell attributes related to malignancy.
Application in cell and molecular biology studies.

More Related Videos

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Related Experiment Videos

Last Updated: Jul 2, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Main Results:

Nucleic acid analysis is a frequent application of flow cytometry.
These methods provide critical information on cell attributes associated with tumor malignancy.
The techniques are versatile and applicable across various research domains.

Conclusions:

Nucleic acid analytical methods are broadly applicable in basic and clinical research.
Flow cytometry-based nucleic acid analysis is a key tool for oncological and molecular studies.
These methodologies enhance the understanding of cell attributes and tumor malignancy.