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Related Experiment Video

Updated: Jul 2, 2026

Chromosome Preparation From Cultured Cells

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

Processing and staining of cell and tissue material for interphase cytogenetics.

A H Hopman¹, F C Ramaekers

¹University of Maastricht, Maastricht, The Netherlands.

Current Protocols in Cytometry

|September 5, 2008

Summary

This study details protocols for preparing biological specimens for in situ hybridization. These methods enable the targeted detection of chromosome aberrations in interphase nuclei.

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Last Updated: Jul 2, 2026

Chromosome Preparation From Cultured Cells

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Published on: January 28, 2014

Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Published on: September 4, 2009

Area of Science:

Molecular Biology
Cytogenetics

Background:

In situ hybridization (ISH) is a powerful technique for visualizing nucleic acid sequences within cells or tissues.
Accurate specimen preparation is critical for successful and reliable ISH results.

Purpose of the Study:

To provide comprehensive protocols for preparing biological specimens for in situ hybridization.
To enable targeted detection of numerical and structural chromosome aberrations in interphase nuclei.

Main Methods:

Detailed protocols for preparing both fresh and fixed biological specimens.
Methods applicable to establishing in situ hybridization procedures using genomic probes.

Main Results:

Established protocols for targeted detection of chromosome aberrations.
Demonstrated applicability across a range of complexity, from simple to advanced.

Conclusions:

The described protocols facilitate robust in situ hybridization for analyzing chromosome abnormalities.
These methods are valuable for research and diagnostic applications in cytogenetics.