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Related Experiment Video

Updated: Jul 2, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

Overview of functional cell assays.

Peter S Rabinovitch1, J Paul Robinson

  • 1University of Washington, Seattle, Washington, USA.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary

Flow cytometry assays measure cell function, not just appearance. These techniques analyze cell viability, metabolism, and other key functional parameters for biological and clinical insights.

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Flow cytometry is a powerful technique for analyzing cellular characteristics.
  • Increasing interest exists in assessing functional cellular parameters beyond simple phenotypic identification.

Purpose of the Study:

  • To review functional assays performed using flow cytometry.
  • To highlight the application of flow cytometry in evaluating diverse cellular functions.

Main Methods:

  • Discussion of various functional assays applicable to flow cytometry.
  • Includes methods for assessing cell viability, oxidative metabolism, membrane potential, intracellular calcium, pH, organelles, and gene reporter systems.

Main Results:

  • Flow cytometry enables the measurement of critical functional parameters.
  • These assays provide insights into the dynamic behavior of cells.

Conclusions:

  • Functional flow cytometry assays offer valuable data for cell biologists and clinicians.
  • These methods expand the utility of flow cytometry beyond phenotype analysis.

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