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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 2, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

Flow cytometry of apoptosis.

Piotr Pozarowski1, Jerzy Grabarek, Zbigniew Darzynkiewicz

  • 1School of Medicine, Lublin, Poland.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary

Flow cytometry is a powerful tool for studying cell death, distinguishing apoptosis from necrosis, and identifying cell death mechanisms. This guide details common methods for analyzing apoptotic indices and introduces a new protocol for detecting serine proteases.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Flow cytometry is crucial for analyzing cell death.
  • Understanding cell death mechanisms is vital in various biological processes.
  • Distinguishing between apoptosis and necrosis is key for accurate cell death studies.

Purpose of the Study:

  • To detail flow cytometric methods for studying cell death.
  • To cover various apoptotic indices and their detection.
  • To introduce a novel protocol for serine protease detection.

Main Methods:

  • Utilizing flow cytometry to analyze cell death.
  • Measuring apoptotic indices such as membrane potential, caspase activation, phosphatidylserine exposure, DNA fragmentation, and transglutaminase activation.

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  • Employing a new FLICA-analogous protocol for serine protease detection.

    • Main Results:

    • Comprehensive overview of established flow cytometry techniques for cell death analysis.
    • Detailed protocols for assessing multiple apoptotic markers.
    • Introduction and validation of a new method for serine protease identification.

    Conclusions:

    • Flow cytometry offers robust methods for cell death research.
    • The presented techniques enable detailed analysis of apoptotic pathways.
    • The novel serine protease detection protocol expands the utility of flow cytometry in cell death studies.