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The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

Meiosis vs. Mitosis

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...

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Related Experiment Video

Updated: Jul 2, 2026

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Detection of mitotic cells.

Gloria Juan¹, Zbigniew Darzynkiewicz

¹Memorial Sloan-Kettering Cancer Center, New York, New York, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

This study details a method to identify and quantify mitotic cells using phosphorylated histone H3 (H3-P) detection. This technique reliably distinguishes dividing cells from apoptotic cells during the G(2) to M transition.

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Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Published on: September 20, 2019

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Nuclear chromatin undergoes significant rearrangement for cell division.
Chromatin condensation, a key event during the G(2) to M transition, is essential for chromosome organization and segregation.
Accurate identification of mitotic cells is crucial for understanding cell cycle progression and its regulation.

Purpose of the Study:

To provide reliable protocols for the identification and quantification of mitotic cells.
To establish a method for detecting histone H3 phosphorylated on Ser 10 (H3-P) as a marker for the G(2) to M transition.
To present an adaptation of the method for multiparameter laser scanning cytometry analysis.

Main Methods:

Immunocytochemical detection of phosphorylated histone H3 (H3-P) using a commercially available antibody.
Concurrent differential staining of cellular DNA to visualize nuclear material.
Adaptation of the immunocytochemical method for cells on microscope slides for laser scanning cytometry.

Main Results:

The H3-P antibody specifically labels mitotic cells and is non-reactive with apoptotic cells.
The protocol allows for accurate identification and quantification of cells undergoing chromatin condensation.
The adapted method enables multiparameter analysis of mitotic cells via laser scanning cytometry.

Conclusions:

Phosphorylation of histone H3 on Ser 10 is a reliable marker for identifying mitotic cells.
The described immunocytochemical protocols provide robust methods for cell cycle analysis.
The technique is applicable to both standard microscopy and advanced flow cytometry techniques.