Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Immunophenotyping of plasma cells.

Andy C Rawstron1

  • 1Leeds Teaching Hospitals, United Kingdom.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary
This summary is machine-generated.

Detecting abnormal plasma cells using flow cytometry can predict myeloma relapse. This method aids in classifying plasma cells for better patient outcome prediction in myeloma and monoclonal gammopathy of undetermined significance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of minimal residual disease (MRD) in salvage autologous stem cell transplantation for relapsed myeloma: results from the NCRI Myeloma X (intensive) trial.

Bone marrow transplantation·2024
Same author

Comprehensive genetic and functional analyses of Fc gamma receptors influence on response to rituximab therapy for autoimmunity.

EBioMedicine·2022
Same author

A Personalized Rituximab Retreatment Approach Based on Clinical and B-Cell Biomarkers in ANCA-Associated Vasculitis.

Frontiers in immunology·2022
Same author

Integrative analysis of spontaneous CLL regression highlights genetic and microenvironmental interdependency in CLL.

Blood·2019
Same author

Ibrutinib Plus Venetoclax in Relapsed/Refractory Chronic Lymphocytic Leukemia: The CLARITY Study.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2019
Same author

Predicting Severe Infection and Effects of Hypogammaglobulinemia During Therapy With Rituximab in Rheumatic and Musculoskeletal Diseases.

Arthritis & rheumatology (Hoboken, N.J.)·2019

Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Plasma cell enumeration and immunophenotyping are crucial for predicting outcomes in myeloma patients.
  • Detection of abnormal plasma cells post-therapy indicates a higher risk of early relapse.
  • The CD138+ plasma cell fraction is the primary indicator of patient outcomes in myeloma.

Purpose of the Study:

  • To establish a four-color flow cytometry protocol for plasma cell enumeration and classification.
  • To differentiate neoplastic from normal plasma cells in diagnostic and post-treatment samples.
  • To correlate plasma cell immunophenotype with myeloma progression risk and patient outcomes.

Main Methods:

  • Utilizing four-color flow cytometry for plasma cell analysis.
  • Identifying plasma cells via strong CD38 and CD138 expression.

More Related Videos

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Related Experiment Videos

Last Updated: Jul 2, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

  • Distinguishing neoplastic plasma cells using aberrant CD19 expression and intracellular immunoglobulin light chain restriction.

    • Main Results:

    • A flow cytometry method for plasma cell enumeration and classification has been developed.
    • Abnormal plasma cells detected above 0.01% at therapy's end predict early relapse.
    • Plasma cell immunophenotype in MGUS correlates with myeloma progression risk.

    Conclusions:

    • This protocol enables accurate plasma cell quantification and classification for myeloma and MGUS.
    • Flow cytometry-based immunophenotyping is vital for predicting patient outcomes and disease progression.
    • The method aids in distinguishing normal from abnormal plasma cells, crucial for treatment monitoring.