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Related Experiment Video

Updated: Jul 2, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Reduced-representation methylation mapping.

Jeffrey A Jeddeloh1, John M Greally, Oliver J Rando

  • 1Roche NimbleGen Inc, Research and Development, 500 S. Rosa Rd, Madison, WI 53719, USA. jeffrey.jeddeloh@roche.com

Genome Biology
|September 6, 2008
PubMed
Summary
This summary is machine-generated.

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Massively parallel sequencing mapped mouse genome cytosine methylation patterns. This revealed links between methylation, DNA sequence, histone modifications, gene activity, and dynamic changes during cell differentiation.

Area of Science:

  • Genomics
  • Epigenetics
  • Molecular Biology

Background:

  • Cytosine methylation is a key epigenetic mechanism regulating gene expression.
  • Understanding methylation patterns is crucial for deciphering cellular processes like differentiation.

Purpose of the Study:

  • To map genome-wide cytosine methylation patterns in the mouse.
  • To investigate the relationship between DNA methylation and other genomic features.

Main Methods:

  • Utilized massively parallel sequencing (next-generation sequencing) for high-resolution mapping.
  • Analyzed methylation patterns in conjunction with DNA sequence, histone modifications, and transcriptional activity data.

Main Results:

  • Successfully mapped extensive cytosine methylation patterns across the mouse genome.

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Methylated DNA Immunoprecipitation
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Methylated DNA Immunoprecipitation

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Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Related Experiment Videos

Last Updated: Jul 2, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

  • Identified correlations between methylation status, DNA sequence context, and histone modification profiles.
  • Observed dynamic changes in methylation patterns during cellular differentiation, linked to transcriptional regulation.

    • Conclusions:

    • Massively parallel sequencing provides powerful insights into epigenetic regulation.
    • Cytosine methylation is intricately linked with DNA sequence, chromatin structure, and gene expression.
    • Methylation dynamics play a significant role in cellular differentiation processes.