Prevalence of Helicobacter pylori in asymptomatic subjects--a nested PCR based study

Area of Science:

• Microbiology
• Infectious Diseases
• Molecular Diagnostics

Background:

• Helicobacter pylori is a significant human pathogen.
• Accurate detection methods are crucial for understanding its prevalence, especially in asymptomatic individuals.
• Nested PCR offers higher specificity compared to serological methods for H. pylori detection.

Purpose of the Study:

• To determine the prevalence of Helicobacter pylori in asymptomatic children and adults.
• To compare the efficacy of nested PCR for H. pylori detection in saliva and stool samples.
• To identify age groups and socioeconomic factors associated with H. pylori infection.

Main Methods:

• Collection of saliva and stool samples from 137 healthy children and 108 asymptomatic adults.
• Utilized nested PCR with primers targeting the Hsp60 gene sequence of H. pylori.
• Analyzed H. pylori positivity rates across different age groups and socioeconomic statuses.

Main Results:

• H. pylori was detected in 45.7% of saliva and 42.8% of stool specimens using nested PCR.
• Prevalence increased with age, peaking in the 11-16 year group, and was higher in individuals of lower socioeconomic status.
• No significant gender bias was observed in H. pylori prevalence.

Conclusions:

• Nested PCR is an effective method for detecting H. pylori in asymptomatic populations.
• H. pylori infection shows a notable prevalence in children and adults, influenced by age and socioeconomic factors.
• Further research into transmission and management strategies for H. pylori in asymptomatic individuals is warranted.