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Updated: Jul 2, 2026

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
Analysis of low molecular weight GTPase activity in endothelial cell cultures
Erika S Wittchen1, Keith Burridge
1Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Abstract:
GTPases control a myriad of cellular processes, including cell migration, proliferation, polarity, and cell adhesion, which includes cell-cell junction regulation. Angiogenesis requires many of these diverse cellular events to occur appropriately. In this chapter, we describe the techniques for in vitro assessment of GTPase activity in endothelial cells grown in monolayer culture as a model system for studying their role during angiogenesis.
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GTPases and their Regulation
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