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Related Experiment Video

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Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

A new perspective on Listeria monocytogenes evolution.

Marie Ragon1, Thierry Wirth, Florian Hollandt

  • 1Institut Pasteur, Laboratoire des Listeria, Paris, France.

Plos Pathogens
|September 6, 2008
PubMed
Summary

Listeria monocytogenes exhibits long-term genetic stability, with distinct clonal complexes correlating to specific serotypes. The virulence factor internalin A (InlA) shows evidence of natural selection and convergent evolution.

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Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Genomics

Background:

  • Listeria monocytogenes is a significant foodborne pathogen and a model organism for host-pathogen interactions.
  • Understanding its evolution is crucial for public health and microbiological research.

Purpose of the Study:

  • To elucidate the phylogenetic structure and evolutionary history of Listeria monocytogenes.
  • To investigate the evolution of virulence factors, specifically internalin A (InlA).

Main Methods:

  • Phylogenetic analysis of 360 isolates using sequencing of seven housekeeping genes (3,288 nucleotides).
  • Multilocus sequence typing (MLST) to define clonal complexes.
  • Bayesian analysis of 438 single nucleotide polymorphisms (SNPs) to reconstruct lineages.
  • Analysis of internalin A (InlA) gene evolution.

Main Results:

  • Seven well-defined clonal complexes were identified, comprising 75% of clinical isolates, each associated with specific serotypes.
  • Limited homologous recombination suggests long-term genetic stability of multilocus genotypes.
  • Serotype 4b evolved from 1/2b, and 1/2c evolved from 1/2a, indicating specific evolutionary pathways.
  • Internalin A (InlA) evolution displayed a mosaic pattern with convergent evolution, suggesting selection for protein truncation.

Conclusions:

  • The study provides a robust evolutionary framework for Listeria monocytogenes.
  • Phylogenetic stability and specific serotype evolution are key features of L. monocytogenes.
  • Convergent evolution of InlA highlights the role of natural selection in virulence factor adaptation.