Related Experiment Video
Updated: Jul 2, 2026

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
Published on: March 21, 2025
Membrane-based techniques for the separation and purification of proteins: an overview
Arunima Saxena1, Bijay P Tripathi, Mahendra Kumar
1Electro-Membrane Processes Division, Central Salt and Marine Chemicals Research Institute, Bhavnagar-364002 Gujarat, India.
Abstract:
Membrane processes are increasingly reported for various applications in both upstream and downstream technology, such as microfiltration, ultrafiltration, emerging processes as membrane chromatography, high performance tangential flow filtration and electrophoretic membrane contactor. Membrane-based processes are playing critical role in the field of separation/purification of biotechnological products. Membranes became an integral part of biotechnology and improvements in membrane technology are now focused on high resolution of bioproduct. In bioseparation, applications of membrane technologies include protein production/purification, protein-virus separation. This manuscript provides an overview of recent developments and published literature in membrane technology, focusing on special characteristics of the membranes and membrane-based processes that are now used for the production and purification of proteins.
More Related Videos
10:33Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
10:08An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
Published on: May 21, 2021
Related Concept Videos
Detergent Purification of Membrane Proteins
Overview Of Cell Separation And Isolation
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Downstream Processing