Related Experiment Video
Updated: Jul 2, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility
Sena Erdogan Aydos1, Mehmet Taspinar, Asuman Sunguroglu
1Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey.
Objective:
To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility.
Design:
Genetic polymorphism analysis, case-control study.
Setting:
University research laboratory and andrology clinic.
Patient(S):
One hundred ten men with infertility and 105 healthy fertile men were recruited for the study.
Intervention(S):
Physical examination of the genitalia of patients, scrotal colored Doppler ultrasound examination, and blood sampling were performed for DNA extraction and genotyping.
Main Outcome Measure(S):
CYP1A1(*)2C, GSTM1, and GSTT1 polymorphism genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism methods. Seminal parameters were analyzed.
Result(S):
There were significant differences between infertility and GSTM1, CYP1A1(*)2C genotypes by univariate analyses. A subject carrying CYP1A1 Val/Val or CYP1A1 Ile/Val in association with GSTM null genotype has 6.90 times more risk to be infertile than a subject carrying CYP1A1 Ile/Ile in association with GSTM1 wild-type genotype (odds ratio: 6.90, 95% confidence interval: 2.29-19.3). No correlation was found between the seminal parameters and the genetic variability.
Conclusion:
Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility.
Related Concept Videos
Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes
Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters
Infertility in Males
Spermatogenesis
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase