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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Published on: July 11, 2016

Intraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing

Marcos R Buim1, Melissa Buzinhani, Maurício Yamaguti

  • 1Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, SP, Brazil.

Comparative Immunology, Microbiology and Infectious Diseases
|September 9, 2008
PubMed

Insights

Mycoplasma synoviae, a poultry pathogen, shows genetic diversity in Brazilian strains. This variation, identified through 16S rRNA gene sequencing, impacts avian health and industry economics.

Area of Science:

  • Avian medicine
  • Microbiology
  • Molecular biology

Background:

  • Mycoplasma synoviae (MS) is a significant avian pathogen causing respiratory and joint diseases in poultry.
  • Economic losses in the Brazilian poultry industry are linked to MS infections.
  • The genotypic variation of MS strains, particularly in the 16S rRNA gene, was previously uncharacterized.

Purpose of the Study:

  • To perform molecular characterization of 19 Mycoplasma synoviae strains from distinct Brazilian poultry production areas.
  • To evaluate the genetic variability within these MS strains using partial 16S rRNA gene sequences.

Main Methods:

  • Sequencing of partial 16S rRNA gene from 19 MS strains.
  • Analysis of polymorphic patterns and nucleotide variations (deletions, insertions, substitutions).

Main Results:

  • Observed different polymorphic patterns among the MS strains.
  • Identified 0 to 6 polymorphic alterations per strain.
  • Detected 3 to 5 nucleotide variations, including deletions, insertions, and substitutions.

Conclusions:

  • The study revealed genotypic diversity among Brazilian Mycoplasma synoviae strains.
  • Spontaneous mutations and flock management practices (culling, repositioning) likely contribute to this genetic diversity.
  • Understanding this variation is crucial for managing MS in poultry production.

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