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Published on: January 25, 2017
Construction of an expression vector for improved secretion of canine IL-18
Kikuya Sugiura1, Takashi Akazawa, Mariko Fujimoto
1Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho Naka-ku, Sakai City, Osaka 599-8531, Japan. sugiura@vet.osakafu-u.ac.jp
Abstract:
To construct a vector for caspase-1 independent expression of canine IL-18, the signal sequence of canine IL-12p40 was fused to the sequence of mature IL-18 on the NdeI restriction site which is located at the 3' end of the signal sequence. The resulting vector expressed coding protein from transfected mammalian cells. The expressed protein was shown to have IL-18 bioactivity in a INF-gamma-inducing assay. These results suggest that the expression vector is the desired tool for advancement of dendritic cell (DC)-based cancer therapy, provided that the vector can successfully be transfected into dendritic cells. We propose a simple and widely applicable method for providing the signal sequence.
