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Rotavirus-associated infantile diarrhea in Uberaba, Minas Gerais, on the wake of the Brazilian vaccination program

A L S Domingues1, A T S Morais, R L Cruz

  • 1Departamento de Ciências Biológicas, Microbiologia,Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil. andredomingues@dcb.uftm.edu.br

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|September 9, 2008
PubMed

Insights

Rotavirus strains G2P[4] and G1P[8] were identified in children with diarrhea in Minas Gerais. This study provides crucial data on rotavirus genotypes following the introduction of a new vaccine.

Area of Science:

  • Virology
  • Pediatrics
  • Public Health

Background:

  • Rotaviruses are a primary cause of childhood diarrhea globally.
  • Vaccines have been developed and implemented to combat rotavirus infections.

Purpose of the Study:

  • To determine the rotavirus genotypes responsible for dehydrating diarrhea in children in Uberaba, Minas Gerais, Brazil.
  • To analyze rotavirus strain dynamics during the initial phase of a national vaccination program.

Main Methods:

  • Fecal specimens were analyzed using Polyacrylamide Gel Electrophoresis (PAGE).
  • Rotavirus strain genotyping was performed using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and PCR-typing.

Main Results:

  • Rotavirus was detected in 15% of fecal samples.
  • The predominant genotype identified was G2P[4] (64%), associated with short electrophoretic patterns.
  • Other identified genotypes included G1P[8], G9P[8], and G4P[9], associated with long electropherotypes.
  • A shift from long to short electrophoretic patterns was observed, coinciding with peak diarrheal incidence.

Conclusions:

  • This study presents the first characterization of circulating rotavirus strains in the Triângulo Mineiro region.
  • Findings were recorded during the first 10 months of Brazil's national rotavirus immunization program, which uses a monovalent G1P[8] vaccine.
  • The data contribute to understanding rotavirus strain dynamics and evaluating the impact of the immunization program.
Abstract

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